In Silico Analysis Validates Proteomic Findings

Written by Ali Azimi
Recently, our original research paper titled “In Silico Analysis Validates Proteomic Findings of Formalin-fixed Paraffin Embedded Cutaneous Squamous Cell Carcinoma Tissue” has been accepted for publication in the journal of Cancer Genomics & Proteomics (CGP). The manuscript will be published in the next available issue of CGP. CGP is an international peer-reviewed journal designed to publish rapidly high-quality articles and reviews on the application of genomic and proteomic technology to basic, experimental and clinical cancer research.
In this paper, we report on proteomic investigations of archival formalin-fixed paraffin embedded (FFPE) cutaneous squamous cell carcinoma (cSCC) material using laser-capture microdissection coupled with mass spectrometry (LC-MS) that could provide reliable biomarkers for the cSCC clinical management. Our study is the first to show that FFPE cSCC tissues offer great opportunity for retrospective proteomic analysis. We identified widespread proteome changes between cSCC and adjacent normal skin. Our study highlights several potential biomarkers of cSCC and reveals a broad spectrum of disrupted signalling pathways that might offer therapeutic targets for early interventions.